Objective To analyze the distribution of respiratory viruses in children with acute respiratory infection in our hospital, so as to provide the basis for clinical diagnosis, treatment and prevention of diseases.Methods Nasopharyngeal swabs from children with acute respiratory infection in Zhongshan Bo'ai Hospital from March 2018 to March 2020 were collected and detected by direct immunofluorescence. Seven kinds of viruses were detected, and the sex, seasonality and age distribution of the results were analyzed. Results A total of 20 264 samples were collected, and the overall positive rate was 20.10%. Among them, the positive rate of RSV was the highest (12.67%), accounting for 60.33% of the total positive results. The lowest positive rate was PIV I (0.07% ). The overall positive rate of respiratoryvirus in malechildren was not significantly different from that in female children (P＞0.05), but the positive rate of ADV in boys (2.78%) was significantly higher than that in girls (1.63%) (P＜0.05). The positive rate of RSV in spring and summer was higher than that in autumn and winter (P＜0.05). The positive rate of PIV Ⅲ in spring and winter was higher than that in summer and autumn (P＜0.05), but there was no significant difference in seasonal distribution of ADV, Flu, PIV Ⅰ and Ⅱ. There were differences in the overall positive rate among different age groups. The positive rate of children aged 0-2 years old was higher than that of other age groups (P＜0.05). Conclusion RSV is the most common virus in children with respiratory infection in our hospital. Sex, seasonality and age can affect the distribution of respiratory virus, we should make targeted prevention and control strategies and means accordingly.