Objective?To evaluate the diagnostic value of non-guided sheath radial ultrasound combined with measurement technique guided transbronchial frozen lung biopsy (R-EBUS-MT-TBCB) in patients with peripheral pulmonary lesions (PPLs). Methods?A total of 180 patients with peripheral pulmonary lesions confirmed by chest CT scan in outpatient department and inpatient department of respiratory medicine of The People's Hospital of Nanhai District from January 2019 to June 2020 were selected as the research objects, and they were randomly divided into observation group 1, observation group 2, and experimental group, 60 cases in each group: Observation group 1 was performed via bronchial forceps biopsy guided by intrabronchial radial ultrasound with guided sheath tube (GS); Observation group 2 underwent bronchial forceps biopsy guided by GS combined intrabronchial radial ultrasound. The test group used R-EBUS-measurement technology -TBCB. The complications of lung biopsy were observed in each group. Average operation time of lung biopsy in each group; Diagnosis rate of lung biopsy in each group. Results?The incidence of lung biopsy complications in experimental group was lower than that in observation group 1 and observation group 2. The mean operation time of lung biopsy in experimental group was less than that in observation group 1 and observation group 2. The accuracy of lung biopsy in experimental group (85.00%) was higher than that in observation group 1 (65.00%) and observation group 2 (70.00%) (P ＜ 0.05). Conclusion?As a potentially better method to diagnose PPLs, R-EBUS-MT-TBCB significantly improves both the safety and the diagnosis rates.